Succinate Transport in Escherichia coli Mutants Defective in Succinate Metabolism
نویسندگان
چکیده
منابع مشابه
The Succinate Dehydrogenase of Escherichia coli
Using EPR spectroscopy to monitor the integrity of the enzyme, conditions have been established which allow specific immunoprecipitation of the succinate dehydrogenase complex of Escherichia coli. The enzyme complex precipitated from Lubrol PX-solubilized membranes by monospecific antiserum in the presence of a cocktail of protease inhibitors contains four polypeptides of apparent Mrs 71,000,26...
متن کاملTransport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells.
A mutant of Escherichia coli unable to metabolize succinate has been used to study the nature of succinate transport in whole cells. It is demonstrated that cells accumulate succinate against a concentration gradient. The uptake of succinate is competitively inhibited by fumarate and malate. The Michaelis constants for the dicarboxylic acids are all in the range of 15 to 30 pM. The accumulation...
متن کاملSATP (YaaH), a succinate-acetate transporter protein in Escherichia coli.
In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM for acetic acid and 1.18±0.10 mM for succini...
متن کاملEscherichia coli mutants defective in dipeptidyl carboxypeptidase.
Two independent mutants of Escherichia coli deficient in dipeptidyl carboxypeptidase activity (Dep-) were isolated after mutagenesis with ethyl methanesulfonate. Mating experiments and introduction of specific episomes indicated that the responsible gene was located at approximately 27--31 min on the E. coli chromosome. The Dep- mutants differed from the parental strain in their inability to gr...
متن کاملTargeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli
BACKGROUND Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering and synthetic biology have been invested for bio-based production of succinate. Precursor phosphoenolpyruvate (PEP) is consumed for transport and phosphorylation of glucose, and...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1972
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.36.2397